Fracture-flip: new high-resolution images of cell surfaces after carbon stabilization of freeze-fractured membranes.
نویسندگان
چکیده
We introduce fracture-flip, a method of obtaining high-resolution views of membrane surfaces. The method, a corollary of label-fracture, is based on the stabilization of the exoplasmic halves of membranes by carbon evaporation. Inversion of these casts followed by Pt/C imaging leads to new views of the cell surface at macromolecular resolution.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of cell science
دوره 90 ( Pt 4) شماره
صفحات -
تاریخ انتشار 1988